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D-Lactate dehydrogenase

D-Lactate dehydrogenase is a diagnostic grade reagent from a microorganism host. This enzyme is useful for enzymatic determination of numerous metabolites, e.g., ATP, ADP, glucose, creatinine, pyruvate, lactate and glycerol, and of enzyme activities, e.g., GPT, PK, and CPK when coupled with the related enzymes.

LD01D1-E311H
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Storage and Stability:

Product can be stored at 2–8°C for transportation process up to ten days but long-term storage should be at -20°C


Activity:

150U/mg-solid or more


Unit Definition:

One unit causes the oxidation of one micromole of NADH per minute at pH 7.0 and 25°C


Grade:

Diagnostic Reagent


EC No.:

EC 1.1.1.28


Appearance:

White amorphous powder, lyophilized


Contaminants:

AST/GOT and ALT/GPT all ≤5.0 × 10-3 %
Malate dehydrogenase ≤1.0 × 10-2 %
NADH oxidase ≤1.0 × 10-3 %
Myokinase ≤1.0 × 10-2 %
Pyruvate Kinase ≤1.0 × 10-3 %


Molecular Weight:

39.0 kDa


Isoelectric Point:

6.1


Michaelis Constant:

6.41×10-4 M (Pyruvate, pH 7.0)


Inhibitors:

Co2+, Fe3+, Cu2+, Ag+, Hg2+, SDS


Optimum pH:

7.0


Optimum Temperature:

40°C


40°C pH Stability:

pH 4.5 – 10.5 (25°C, 20 hr)


Thermal Stability:

Below 50°C (pH 7.0, 15 min)




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