LsCsm6

Alternate Names:

CRISPR system endoribronuclease Csm6, CRISPR type III-A associated protein Csm6

Overview:

LsCsm6 is a single-stranded RNA-specific endoribonuclease that consists of the N-terminal CARF domain and the C-terminal HEPN domain. The ribonuclease active site is formed by dimerization of the HEPN domains. LsCsm6 belongs to the Class 1 type-III-A CRISPR-Cas system (1). Upon activation, the CRISPR ancillary ribonucleases Csx1/Csm6, the HEPN active site degrades invasive RNA in a non-specific manner. LsCsm6 is activated by cyclic oligoadenylate second messengers synthesized from ATP by the Cas10 subunit of the Type III interference complex (2).

Accession Number:

WP_081509150

Formulation:

Protein stored in 25 mM Tris, 300 mM NaCl, pH 7.5, 0.1 mM TCEP, 50% glycerol

References:

1. Niewoehner, O. et al. 2016. Structural basis for the endoribonuclease activity of the type III-A CRISPR-associated protein Csm6. RNA 22(3):318-329.
2. Niewoehner, O. et al. 2017. Type III CRISPR–Cas systems produce cyclic oligoadenylate second messengers. Nature 548(7669): 543-548.

Storage and Stability:

Store at –20°C. To avoid repeated handling and multiple freeze/thaw cycles, aliquot product into smaller quantities.

Concentration:

1.0 μg/μl
20.4 pmol/μg

Molecular Weight:

Calculated: 49.6 kDa
Observed: ~49 kDa

Purity:

The purity was determined to be >90% by densitometry

Recommendation:

Always include RNase inhibitor at recommended concentrations in Cas enzyme reactions.