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Lactate Oxidase

Lactate Oxidase is a diagnostic grade reagent from a microorganism host. This enzyme is useful for enzymatic determination of L-lactate in clinical analysis.

LX01D1-E311H
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Storage and Stability:

Product can be stored at 2–8°C for transportation process up to ten days but long-term storage should be at -20°C


Activity:

80 U/mg-solid or more


Unit Definition:

One unit causes the formation of one micromole of hydrogen peroxide (half a micromole of quinoneimine dye) per minute at pH 7.5 and 37°C.


Grade:

Diagnostic Reagent


EC No.:

EC 1.13.12.4


Appearance:

Yellowish amorphous powder, lyophilized


Molecular Weight:

42.0 kDa


Contaminants:

Pyruvate oxidase ≤1.0 ×10-3 %
Cholesterol oxidase ≤1.0 × 10-3 %
Uricase ≤1.0 ×10-3 %
Glucose oxidase ≤1.0 × 10-3 %


Isoelectric Point:

5.75


Michaelis Constant:

1.0 ×10-3 M (L-Lactate)


Inhibitors:

Fe3+, Ag+, Hg2+, SDS


Optimum pH:

7.5


Optimum Temperature:

30 ~ 35°C


pH Stability:

pH 5.5–10.5 (25°C, 16 hr)


Thermal Stability:

Below 60°C (pH 7.0, 10 min)




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