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Purine-Nucleoside Phosphorylase

This enzyme is a diagnostic grade reagent from a microorganism host. It is useful for enzymatic determination of inorganic phosphate, 5’-nucleotidase and adenosine deaminase when coupled with xanthine oxidase and uricase.

PP01D1-E311H
Bulk

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Storage and Stability:

Product can be stored at 2~8°C for transportation process up to ten days but long-term storage should be at –20°C.


Activity:

30U/mg-solid or more


Unit Definition:

One unit causes the formation of one micromole of uric acid per minute at pH 7.7 at 37°C.


Applications:

It is useful for enzymatic determination of inorganic phosphate, 5’-nucleotidase and adenosine deaminase when coupled with xanthine oxidase and uricase.


EC No.:

EC 2.4.2.1


Grade:

Diagnostic Reagent


Appearance:

White amorphous powder, lyophilized


Contaminants:

Catalase ≤20%
ATPase ≤1.0 ×10-2 %
5’-Nucleosidase & Adenosine deaminase ≤1.0 ×10-3 %


Molecular Weight:

30 kDa


Isoelectric Point:

5.83


Michaelis Constant:

7.33 ×10-5M (Inosine), 1.05 ×10-4M (Pi)


Inhibitors:

Co3+, Cu2+, Hg2+, Ag+, SDS, NEM, IAA, ProClin-300


Optimum pH:

6.5-7.5


Optimum Temperature:

55~65°C


pH Stability:

pH 6.0~10.5 (30°C, 16 hr)


Thermal Stability:

below 65°C (pH 7.7, 30 min)




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