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rRNA Depletion Kit

SignalChem Diagnostics Human rRNA Depletion Kit removes ribosomal RNA and 45S ribosomal precursor internal and external transcribed spacer (ITS/ETS) sequences. The kit uses RNase H to allow non-ribosomal RNA such as mRNA to be retained for downstream RNA sequencing analysis. It is compatible with total RNA isolated from humans, mice, and rats, and is effective for both high-quality intact RNA as well as degraded material, such as RNA extracted from FFPE tissues.

This kit accommodates RNA inputs ranging from 100 ng to 1 μg, and consistently removes > 95% of rRNA, ITS, and ETS sequences. The resulting RNA produced from this kit can be used for various applications, including gene expression, alternative splicing, identification of non-coding RNA, analysis of polyadenylation sites, and fusion gene detection research.

RD01-K29A
12 T24 T96 T Bulk Customize

$ 475

*The image is illustrative and does not necessarily represent the product


Storage and Stability:

Store at –25 °C ~ -15 °C for 1 year


Components:

Hybridization Buffer (green label) : 36 μL (12 T); 72 μL (24 T); 288 μL (96 T)
Human Probe Mix (rRNA and ITS/ETS) (green label) : 24 μL (12 T); 48 μL (24 T); 192 μL (96 T)
RNase H Buffer (purple label) : 36 μL (12 T); 72 μL (24 T); 288 μL (96 T)
RNase H (purple label) : 192 μL (12 T); 192 μL (24 T); 192 μL (96 T)
DNase I Buffer (white label) : 330 μL (12 T); 660 μL (24 T); 2 x 1320 μL (96 T)
DNase I (white label): 30 μL (12 T); 60 μL (24 T); 240 μL (96 T)


Additional Information:

1. To prevent RNase contamination, use RNase-free consumables and clean the experimental area with nucleic acid removal.
2. Ensure there is no gDNA residue in the sample by using DNase I before this kit.
3. Please concentrate sample to a maximum input volume of 10 μL.
4. For research use only.
5. Use appropriate personal protective equipment (PPE), such as a laboratory coat and gloves, and follow proper disposal protocols.




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