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Sarcosine Oxidase

This enzyme is a diagnostic grade reagent from a microorganism host. It is useful for enzymatic determination of creatinine, creatine and sarcosine when coupled with creatinine amidohydrolase and creatine amidinohydrolase in clinical analysis.

SX01D1-E311H
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Storage and Stability:

Product can be stored at 2~8°C for transportation process up to ten days but long-term storage should be at –20°C.


Activity:

10 U/mg-solid or more


Unit Definition:

One unit causes the formation of one micromole of hydrogen peroxide (half a micromole of quinoneimine dye) per minute at pH 8.0 and 37°C.


Grade:

Diagnostic Reagent


EC No.:

EC 1.5.3.1


Appearance:

Yellow amorphous powder, lyophilized


Molecular Weight:

44.0 kDa


Contaminants:

Catalase ≤1.0 %


Stabilizers:

Sucrose


Isoelectric Point:

Isoelectric Point


Michaelis Constant:

6.938 ×10-3 M (sarcosine)


Inhibitors:

Cu2+, Ag+, Hg2+, NEM


Optimum pH:

7.5


Optimum Temperature:

55 ~ 60°C


pH Stability:

pH 6.5–9.5 (25°C, 24 hr)


Thermal Stability:

Below 55°C (pH 7.5, 30 min)




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